FGF10(F830-40N)

FGF10(F830-40N)

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Description :Recombinant Human FGF10 was expressed in E. coli cells.

Species :Human

Expression System:E.coli

Genbank Number :O15520

Storage, Stability and Shipping :Storage The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight :19.0 kDa

Gene Aliases :FGF-10, FGFA, Keratinocyte growth factor-2
Recombinant Human Fibroblast Growth Factor 10 (FGF10)

Reconstitution Protocol :A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Scientific Background :FGF-10 is involved in the initial budding as well as the continuous outgrowth of vertebrate limbs. FGF-10 mRNA is expressed preferentially in neurons but not in glial cells and may have a distinct role in the brain. Human FGF-10 is mitogenic for fetal rat keratinizing epidermal cells but not for NIH 3T3 cells. Recombinant FGF-10 induces the proliferation of human urothelial cells in vitro and induces the proliferation of transitional epithelium. FGF-10 is secreted by cultured mouse pre- adipocytes, prevention of FGF-10 signaling inhibits subsequent differentiation. The ability of embryonic fibroblasts derived from FGF-10 knock-out mice to differentiate into adipocytes is also impaired.

Sample Cell Assay Data :The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05 - 0.5 ng/ml.

Purity :Recombinant Human FGF10 resolved on a 15% SDS_PAGE gel under reducing conditions and stained with Coomassie Blue G-250.

Product Sheets (By Lot #) :

G3211-30.pdf

Research Areas :