Anti-phospho-mTOR (Ser2448)(F17-65R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the mTOR protein phosphorylated at serine 2448
Cited Applications :IHC
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :The antiserum was produced against synthesized phosphopeptide derived from human mTOR around the phosphorylation site of serine 2448 (T-D-SP-Y-S).
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-mTOR (Ser2448).
Scientific Background :The mammalian target of rapamycin, mTOR, also known as FRAP or RAFT, is a Ser/Thr protein kinase. mTOR acts as a sensor for ATP and amino acids, balancing the availability of nutrients and cell growth (1). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal, transmits a positive signal to p70 S6 kinase and participates in the inactivation of the eIF4E inhibitor, 4E-BP1 (2).
References :
1. Sabers CJ et al: Isolation of a protein target of the FKBP12-rapamycin complex in mammalian cells. J Biol Chem. 270(2): 815-22, 1995.
2. Fang Y. et al: Phosphatidic acid-mediated mitogenic activation of mTOR signaling. Science. 294(5548): 1942-5, 2001.
3. Holz MK, et,al. (2005) J Biol Chem ;280:26089-26093.
4. Chiang GG, et,al. (2005) J Biol Chem ;280: 25485-25490.
5. Mothe-Satney I, et,al. (2004) J Biol Chem ;279: 42628-42637.
6. Bolster DR, et,al. (2003) J Physiol ;553:213-220.
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Research Areas :AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Metabolic Disorder, Cancer, Inflammation, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, Apoptosis/Autophagy, Angiogenesis