FLT3 (Y591-V592insVDFREYEYD D835Y), Active(F12-12JG)
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Description :Recombinant human FLT3 (Y591-V592insVDFREYEYD D835Y) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. This FLT3-ITD mutant has 9 amino acids “VDFREYEYD” repeated between the sites Y591 and V592 within the juxtamembrane domain.
Species :Human
Tag :GST
Expression System:Sf9 insect cells using baculovirus
Sequence :
Genbank Number :NM_004119
Alternative Name(s) :FLK2, STK1, CD135
Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :Fms-related tyrosine kinase 3 (FLT3) is a gene that encodes for a tyrosine kinase that activates pathways in hematopoietic cells important in cellular proliferation. FLT3 is frequently mutated in acute myeloid leukemia (AML), myelodysplastic syndromes, other hematologic malignancies, and colorectal cancer. FLT3 mutations are observed in 24.3% of AML, of which internal tandem duplications (ITD) are the most frequent. FLT3 ITD occurs when sequences of less than ten to several hundred bases in length are repeated within the juxtamembrane domain are always “in-frame”.
References :1. http://www.mycancergenome.org/content/disease/acute-myeloid-leukemia/flt3/277/
2. Fathi AT, et al: Treatment of FLT3-ITD acute myeloid leukemia. Am J Blood Res. 1(2):175-89, 2011
Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Observed MW ~76 kDa. Calculated MW ~75 kDa.
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