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Description :Recombinant full-length human EZH1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :BC015882
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~130 kDa
Gene Aliases :KIAA0388
Scientific Background :EZH1 (enhancer of zeste homolog 1) belongs to the polycomb group (PcG) protein and acts as the catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of target genes. EZH1 is ubiquitously expressed. Compared to PRC2-EZH2, PRC2-EZH1 exhibits low levels of histone methyltransferase (HKMT) activity. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity.
1. Margueron R, et al: Ezh1 and Ezh2 maintain repressive chromatin through different mechanisms. Mol Cell. 32(4):503-18, 2008.
2. Shen X, et al: EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency. Mol Cell. 32(4):491-502, 2008.
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Research Areas :Cancer, Neurobiology, Inflammation