EHMT1 (KMT1D), Active(E317-381G)
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Description :Recombinant human EHMT1 (895-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Genbank Number :NM_024757
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Methyltransferase Assay
Molecular Weight :74 kDa
Gene Aliases :GLP; GLP1; Eu-HMTase1; EUHMTASE1; FP13812; KMT1D; bA188C12.1
Scientific Background :Euchromatic histone-lysine N-methyltransferase 1 (EHMT1) is in charge of the mono- and demethylation of lysine-9 of histone H3. EHMT1 is found in a multimeric protein complex containing chromatin modifiers, which contributes to silencing of MYC- and E2F-responsive genes during G0 phase of cell cycle (1). EHMT1 plays a role in epithelial-to-mesenchymal transition and tumor invasion by repressing expression of tumour suppressor genes (2). Additionally, EHMT1 also methylates non-histone proteins such as lysine-373 of p53 (3).
1. Ogawa, H., et al. A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells. Science. 296(5570):1132-6, 2002.
2. Kokura, K., et al. Methyl-H3K9-binding protein MPP8 mediates E-cadherin gene silencing and promotes tumour cell motility and invasion. EMBO J. 29(21):3673-87, 2010.
3. Huang, J., et al. G9a and Glp methylate lysine 373 in the tumor suppressor p53. J Biol Chem. 285(13):9636-41, 2010.
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Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle, Neurobiology