EK (enterokinase), Active(E535-31H)

EK (enterokinase), Active(E535-31H)

  • $270.00


Description :Recombinant swine EK (800-end) was expressed in yeast cells using an N-terminal His tag.

Species :Swine

Tag :His tag

Expression System:Yeast cells

Sequence :800-end

Sample Data :

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Enzyme Commission Number :EC

Gene Aliases :Enteropeptidase; TMPRSS15; PRSS7; Serine protease 7; ENTK; MGC133046.

Storage, Stability, and Shipping :Store product at –20oC for up to 1 year. Aliquot enzymes to avoid freeze / thaw cycles.

Applications :In vitro Proteolysis

Scientific Background :Enterokinase (EK) is a member of the trypsin family of serine proteases, which cleaves proteins following the Lys at the Asp-Asp-Asp-Asp-Lys recognition sequence. In the stomach, EK converts trypsinogen into trypsin. EK has high specificity and high hydrolytic efficiency, making it a widely used biochemical tool for cleaving recombinant fusion proteins. EK can tolerate a variety of detergents and some denaturing agents. EK’s activity is reduced by > 250 mM NaCl, > 2 M urea, > 20 mM, β-ME, > 0.1% SDS and > 250 mM imidazole.

Product Sheets (By Lot #) :