EK (enterokinase), Active(E535-31H)
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Description :Recombinant swine EK (800-end) was expressed in yeast cells using an N-terminal His tag.
Species :Swine
Tag :His tag
Expression System:Yeast cells
Sequence :800-end
Sample Data :
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Enzyme Commission Number :EC 3.4.21.9
Gene Aliases :Enteropeptidase; TMPRSS15; PRSS7; Serine protease 7; ENTK; MGC133046.
Storage, Stability, and Shipping :Store product at –20oC for up to 1 year. Aliquot enzymes to avoid freeze / thaw cycles.
Applications :In vitro Proteolysis
Scientific Background :Enterokinase (EK) is a member of the trypsin family of serine proteases, which cleaves proteins following the Lys at the Asp-Asp-Asp-Asp-Lys recognition sequence. In the stomach, EK converts trypsinogen into trypsin. EK has high specificity and high hydrolytic efficiency, making it a widely used biochemical tool for cleaving recombinant fusion proteins. EK can tolerate a variety of detergents and some denaturing agents. EK’s activity is reduced by > 250 mM NaCl, > 2 M urea, > 20 mM, β-ME, > 0.1% SDS and > 250 mM imidazole.
Product Sheets (By Lot #) :