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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the eIF4E protein

Cited Applications :WB

Cross Reactivity :Western blot eIF4E from human and mouse cells

eIF4E from other species may also be detectable.

Host :Rabbit

Immunogen :Synthesized peptide corresponds to amino acid 209 of human eIF4E protein.

Purification :Affinity Chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Representative western blot with Anti-eIF4E (1:1000) using 20 μg of Hela, 293, THP1 and 3T6 cell lysates.

Scientific Background :EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. EIF4E and is the rate-limiting component in protein synthesis. Overexpression of EIF4E causes malignant transformation (1). Overexpression of EIF4E in transgenic mice causes a marked increase in tumorigenesis. EIF4E is a potential physiological substrate for MNK1 and MNK2. MNK1 interacts with the EIF4F complex via its interaction with the C-terminal region of EIF4G, not through a direct interaction with EIF4E (2).

References :
1. Ruggero D, et al: The translation factor eIF-4E promotes tumor formation and cooperates with c-Myc in lymphomagenesis. Nature Med. 10: 484-486, 2004.

2. Waskiewicz A J, et al: Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2. EMBO J. 16: 1909-1920, 1997.

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Research Areas :Cancer, Neurobiology, Inflammation, ERK/MAPK Pathway, Cell Cycle, Cellular Stress, Angiogenesis, Cancer, Neurobiology, Inflammation, ERK/MAPK Pathway, Cell Cycle, Cellular Stress, Angiogenesis