Anti-eIF4E(E34-63R)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the eIF4E protein
Cited Applications :WB
Cross Reactivity :Western blot eIF4E from human and mouse cells
eIF4E from other species may also be detectable.
Host :Rabbit
Immunogen :Synthesized peptide corresponds to amino acid 209 of human eIF4E protein.
Purification :Affinity Chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot with Anti-eIF4E (1:1000) using 20 μg of Hela, 293, THP1 and 3T6 cell lysates.WBgel.jpg)
Scientific Background :EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. EIF4E and is the rate-limiting component in protein synthesis. Overexpression of EIF4E causes malignant transformation (1). Overexpression of EIF4E in transgenic mice causes a marked increase in tumorigenesis. EIF4E is a potential physiological substrate for MNK1 and MNK2. MNK1 interacts with the EIF4F complex via its interaction with the C-terminal region of EIF4G, not through a direct interaction with EIF4E (2).
References :
1. Ruggero D, et al: The translation factor eIF-4E promotes tumor formation and cooperates with c-Myc in lymphomagenesis. Nature Med. 10: 484-486, 2004.
2. Waskiewicz A J, et al: Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2. EMBO J. 16: 1909-1920, 1997.
Product Sheets (By Lot #) :
Research Areas :Cancer, Neurobiology, Inflammation, ERK/MAPK Pathway, Cell Cycle, Cellular Stress, Angiogenesis, Cancer, Neurobiology, Inflammation, ERK/MAPK Pathway, Cell Cycle, Cellular Stress, Angiogenesis