HER3 Protein(E28-35H)

HER3 Protein(E28-35H)

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Description :Recombinant human HER3 (667-1020) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Species :Human

Tag :HIS

Expression System:Sf9 insect cells using baculovirus

Sequence :667-1020

Genbank Number :P21860

Alternative Name(s) :ERBB3

Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.

Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background :HER3 is a member of the human epidermal growth factor receptor (EGFR/HER) tyrosine kinase family whose kinase domain lacks several residues critical for catalysis but serves as an activator of the EGFR kinase domain (1,2). Phosphorylation of HER3 by other EGFR family members, most commonly HER2, activates the PI3K/AKT pathway (2). In cancer cells treated with HER2 kinase inhibitors, diminished HER2 activity is compensated for by HER3 overexpression, resulting in tumor cell survival (2).

References :1. Shi, F. et al: ErbB3/HER3 intracellular domain is competent to bind ATP and catalyze autophosphorylation. Proc Natl Acad Sci USA 107(17): 7692-7697, 2010.
2. Jura, N. et al: Structural analysis of the catalytically inactive kinase domain of the human EGF receptor 3. Proc Natl Acad Sci USA 106(51): 21608-21613, 2009.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Molecular Weight :Calculated MW ~41 kDa.

Product Sheets (By Lot #) :

T4276-15.pdf

Research Areas :Cancer, Cell Cycle, Cellular Stress, Receptor Tyrosine Kinases