HER2 (A775_G776insYVMA C805S), Active(E27-132FG)

HER2 (A775_G776insYVMA C805S), Active(E27-132FG)

  • $226.00


FOR BULK ORDER REQUESTS PLEASE CONTACT US

Description :Recombinant human HER2 (A775_G776insYVMA C805S) (676-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST

Expression System:Sf9 insect cells using baculovirus

Sequence :676-end (A775_G776insYVMA C805S)

Genbank Number :NM_004448

Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Alternative Name(s) :NEU; NGL; HER2; TKR1; ERBB2; c-erb B2; HER-2/neu

Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.

Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background :HER2 gene encodes a cell-surface glycoprotein tyrosine kinase receptor with extensive homology to the epidermal growth factor receptor. HER2 is an oncogene and overexpression of unaltered HER2 coding sequences in NIH 3T3 cells results in cellular transformation and tumorigenesis (1). HER2 is amplified in about 30% of primary human breast malignancies and overexpression of HER2 is associated with the most aggressive tumors that show uncontrolled proliferation, resistance to apoptosis and increased motility (2).

References :1. Hudziak, R M. et al: Increased expression of the putative growth factor receptor p185HER2 causes transformation and tumorigenesis of NIH 3T3 cells. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7159-63.
2. Badache, A. et al: The ErbB2 Signaling Network as a Target for Breast Cancer Therapy. J Mammary Gland Biol Neoplasia. 2006 Jan; 11(1):13-25.

Molecular Weight :Observed MW ~116 kDa. Calculated MW ~92 kDa.

Product Sheets (By Lot #) :

A4661-22.pdf

U4773-8

Research Areas :Cancer, Cell Cycle