EIF2AK3, Active(E11-11G)
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Description :Recombinant human EIF2AK3 (563-1115) was expressed in E. coli cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:E.coli
Sequence :563-1115
Genbank Number :NM_004836
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~115 kDa
Gene Aliases :PERK, PEK, WRS, HRI, DKFZp781H1925
Scientific Background :EIF2AK3 phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2) leading to its inactivation and a rapid reduction of translational initiation and repression of global protein synthesis. EIF2AK3 is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins (1). EIF2AK3 plays a major role in the ability of cells to adapt to ER stress and is also involved in an integrated adaptive response to hypoxic stress in HeLa cells (2). EIF2AK3 functions in iron homeostasis and may play a role in hemolytic and inflammatory anemia.
References :
1. Harding, H. P. et.al: EIF2AK3 is essential for translational regulation and cell survival during the unfolded protein response. Molec. Cell 5: 897-904, 2000.
2. Blais, J. D. et.al: Activating transcription factor 4 is translationally regulated by hypoxic stress. Molec. Cell. Biol. 24: 7469-7482, 2004.
Product Sheets (By Lot #) :
Research Areas :Angiogenesis, Cellular Stress, Inflammation, Neurobiology, Ser/Thr Kinases, Neurobiology, Inflammation, Cellular Stress, Angiogenesis, Ser/Thr Kinases