EGFR (V948R), Active(E10-122H3H)

EGFR (V948R), Active(E10-122H3H)

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Description :Recombinant human EGFR (V948R) (669-1022) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Species :Human

Tag :HIS

Expression System:Sf9 insect cells using baculovirus

Sequence :669-1022

Genbank Number :NM_005228

Alternative Name(s) :ERBB, mENA, ERBB1, HER1

Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.

Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background :EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival (1). Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms (2). Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation. The V948R mutation is at the active dimer interface, resulting in an enzyme maintained in a monomeric state.

References :1. Wang K, et al: Epidermal growth factor receptor-deficient mice have delayed primary endochondral ossification because of defective osteoclast recruitment. J. Biol. Chem. 279: 53848-53856, 2004.
2. Kobayashi S, et al: EGFR mutation and resistance of non-small-cell lung cancer to gefitinib. New Eng. J. Med. 352: 786-792, 2005.

Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Molecular Weight :Observed MW ~48 kDa.

Product Sheets (By Lot #) :

T4288-17.pdf

Research Areas :Cancer, Cell Cycle