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Description :Recombinant full-length human DNMT3B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :BC111933
Specific Activity :Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Methyltransferase Assay
Molecular Weight :~138 kDa
Gene Aliases :ICF; ICF1; M.HsaIIIB
Scientific Background :DNMT3B or DNA (cytosine-5-)-methyltransferase 3 beta encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. DNMT3B can methylate unmethylated and hemimethylated DNA with equal efficiencies (1). DNMT3B localizes primarily to the nucleus and its expression is developmentally regulated and is required for genome wide de novo methylation and is essential for mammalian development (2). Mutations in DNMT3B casue the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.
1. Yanagisawa, Y. et al: The human DNA methyltransferases DNMT3A and DNMT3B have two types of promoters with different CpG contents. Biochim. Biophys. Acta 1577: 457-465, 2002.
2. Okano, M. et al: DNA methyltransferase Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99: 247-257, 1999.
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Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle, Neurobiology, Cancer, Neurobiology, Apoptosis/Autophagy, Cell Cycle