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DHH(D834-40N)

DHH(D834-40N)

  • $160.00


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Description :Recombinant Human DHH was expressed in E. coli cells.

Species :Human

Expression System:E.coli

Genbank Number :O43323

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight :20.0 kDa

Gene Aliases :Desert Hedgehog Protein, DHH
Recombinant Human Desert Hedgehog (DHH)

Reconstitution Protocol :Reconstitute at 500 μg/mL in sterile PBS

Scientific Background :The Desert Hedgehog protein is one of the three Hedgehog family proteins that is highly conserved and plays a role in many developmental processes in animals. DHH become biologically active following its autocatalytic cleavage. There is a high degree of residue homology between Sonic Hedgehog (SHH), Indian Hedgehog (IHH), and DHH. Despite their individual levels of expression and unique biological roles, the Hedgehog family members all use the same signalling pathway, and therefore, may be used interchangeably for some experimental purposes.

Sample Cell Assay Data :Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 15­-45 µg/Ml.

Purity :Recombinant Human DHH resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.

Product Sheets (By Lot #) :

G3211-25.pdf

Research Areas :Cancer