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DCAMKL1, Active(D14-11G)

DCAMKL1, Active(D14-11G)

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Description :Recombinant human DCAMKL1 (266-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :266-end

Genbank Number :NM_004734

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~80 kDa

Gene Aliases :DCLK1; DCDC3A; DCLK; KIAA0369

Scientific Background :DCAMKL1 or doublecortin-like kinase 1 contains two N-terminal doublecortin domains (which bind microtubules and regulate microtubule polymerization), a C-terminal serine/threonine protein kinase domain (which shows substantial homology to Ca2+/calmodulin-dependent protein kinase), and a serine/proline-rich domain in between the doublecortin and the protein kinase domains (which mediates multiple protein-protein interactions) (1). DCAMKL1 is a microtubule-associated kinase that can undergo autophosphorylation. DCAMKL1 has microtubule-polymerizing activity that is independent of its protein kinase activity (2). DCAMKL1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis.

References :
1. Ohmae, S. et.al: Molecular identification and characteri-zation of a family of kinases with homology to Ca(2+) /calmodulin-dependent protein kinases I/IV. J. Biol. Chem. 281: 20427-20439, 2006.

2. Lin, P. T. et,al: DCAMKL1 encodes a protein kinase with homology to doublecortin that regulates microtubule polymerization. J. Neurosci. 20: 9152-9161, 2000.

Product Sheets (By Lot #) :

U1618-5.pdf

Q2594-8.pdf

Research Areas :Cardiovascular Disease, Ser/Thr Kinases, PKA/PKC Pathway, Neurobiology, ERK/MAPK Pathway