Anti-DOK1(D42-63R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the DOK1 protein
Cited Applications :WB
Cross Reactivity :Western blot DOK1 from human and mouse cells
DOK1 from other species may also be detectable.
Host :Rabbit
Immunogen :Synthetic peptide corresponding to amino acids 360-364 of human DOK1 (Q99704)
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot with Anti-DOK1 (1:1000) using 3 μg, 10 μg and 30 μg of 3T6 cell lysate.
Scientific Background :DOK1 contains pleckstrin homology domain and ten PXXP SH3 recognition motifs and is a critical substrate for p210 bcr/abl. DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors cells isolated from CML patients (1). DOK1 is associated with p120 Ras-GAP and this association is correlated with its tyrosine phosphorylation. Aberrant phosphorylation of DOK1 may contribute to the progression of different human diseases (2).
References :
1. Carpino, N. et.al: p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells. Cell 88: 197-204, 1997.
2. Yasuda. et.al: Role of Dok-1 and Dok-2 in myeloid homeostasis and suppression of leukemia. J. Exp. Med. 200: 1681-1687, 2004.
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Research Areas :Cancer, ERK/MAPK Pathway, Cancer, ERK/MAPK Pathway