DGKG, Active (D25-10BH)

DGKG, Active (D25-10BH)

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Description : Recombinant full-length isoform 1 of human Diacylglycerol kinase gamma (DGKG) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Species : Human

Tag:  His tag

Expression System:  Sf9 insect cells using baculovirus

Sequence:  Full length

Genbank Number:  NM_001346.3

Specific Activity : Sample Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications : Kinase Assay

Molecular Weight :  97 kDa

Gene Aliases : Diglyceride kinase gamma, DGKgamma, DAGK3, DAGKgamma

Formulation : Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.

Scientific Background: Diacylglycerol kinase gamma (DGKG) is a member of DGK type I subfamily which have EF-hand motifs and two cysteine-rich regions (C1 domain) in the regulatory domain. These enzymes catalyze the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins such as protein kinase C isoforms and RAS guanyl nucleotide-releasing proteins and some transient receptor potential channels (1). DGKG has been associated with malignancies with its role as tumor suppressor in colorectal cancer (2). DGKG is also implicated in regulation of cell cycle regulation (3).

References : 

  1. Sakane, F. et al. (1997) Diacylglycerol kinase J. Biol. Chem. 29, 1139-1143.
  2. Kai, M.et al (2017). Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer. Mol. Carcinog. 56, 1743-1752.
  3. Matsubara, T. et al. (2006). Nuclear transportation of diacylglycerol kinase γ and its possible function in the nucleus. J. Biol. Chem. 281, 6152-6164

Product Sheets: (by Lot #):

 

J3872-2

 

Research Areas: Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Inflammation, PKA/PKC Pathway,