DGKA, Active, (D21-10FH)
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Description : Recombinant full-length mouse DGKA was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Species : Mouse
Tag: HIS tag
Expression System: Sf9 insect cells using baculovirus
Sequence: Full length
Genbank Number: NM_016811.
Specific Activity : Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping : Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight : 83 kDa
Gene Aliases : AW146112; Dagk1
Formulation : Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
Scientific Background : Diacylglycerol kinase alpha (DAG kinase alpha) is a member of the eukaryotic diacylglycerol kinase family. Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resyn-thesis of phosphatidylinositols and attenuating protein kinase C activity. Involved in hepatocellular carcinoma progression through regulation of the Ras/Raf/MEK/ERK pathway. The DGKα/atypical PKC/β1 integrin signaling pathway is required for matrix invasion of breast carcinoma cells.
References :
- Takeishi, K. et al. (2012). Diacylglycerol kinase alpha enhances hepatocellular carcinoma progression by activation of Ras-Raf-MEK-ERK pathway. J. Hepatol. 57, 77–83.
- Rainero, E. et al. (2014). The diacylglycerol kinase alpha/atypical PKC/beta1 integrin pathway in SDF-1alpha mammary carcinoma invasiveness. PLoS ONE 9:e97144.
- Sakane F, et al. (2016). Diacylglycerol Kinases as Emerging Potential Drug Targets for a Variety of Diseases: An Update. Front Cell Dev Biol. 4:82.
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Research Areas: Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Inflammation, PKA/PKC Pathway,