• $595.00


Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the DCAMKL1 protein

Cited Applications :WB, ELISA, IF, IHC

Cross Reactivity :Human, Mouse and Rat

Host / Isotype / Clone# :Rabbit, IgG

Immunogen :DCAMKL1 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human DCAMKL1

Purification :Affinity chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot analysis of DCAMKL1 in human brain tissue lysate with DCAMKL1 antibody at (A) 0.5 and (B) 1 ug/ml.

Sample Data :Immunohistochemistry of DCAMKL1 in rat brain tissue with DCAMKL1 antibody at 2.5 ug/ml.

Scientific Background :DCAMKL1 or doublecortin-like kinase 1 contains two N-terminal doublecortin domains (which bind microtubules and regulate microtubule polymerization), a C-terminal serine/threonine protein kinase domain (which shows substantial homology to Ca2+/calmodulin-dependent protein kinase), and a serine/proline-rich domain in between the doublecortin and the protein kinase domains (which mediates multiple protein-protein interactions) (1). DCAMKL1 is a microtubule-associated kinase that can undergo autophosphorylation. DCAMKL1 has microtubule-polymerizing activity that is independent of its protein kinase activity (2). DCAMKL1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis.

References :
1. Ohmae, S. Molecular identification and characterization of a family of kinases with homology to Ca(2+)/calmodulin-dependent protein kinases I/IV. J. Biol. Chem. 281: 20427-20439, 2006

2. Lin, P. T. et,al: DCAMKL1 encodes a protein kinase with homology to doublecortin that regulates microtubule polymerization. J. Neurosci. 20: 9152-9161, 2000.

Product Sheets (By Lot #) :


Research Areas :Cardiovascular Disease, Neurobiology, PKA/PKC Pathway, Ser/Thr Kinases, Neurobiology, Cardiovascular Disease, PKA/PKC Pathway, Ser/Thr Kinases