Anti-DCAMKL1(D14-63R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the DCAMKL1 protein
Cited Applications :WB, ELISA, IF, IHC
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :DCAMKL1 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human DCAMKL1
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of DCAMKL1 in human brain tissue lysate with DCAMKL1 antibody at (A) 0.5 and (B) 1 ug/ml.
Sample Data :Immunohistochemistry of DCAMKL1 in rat brain tissue with DCAMKL1 antibody at 2.5 ug/ml.
Scientific Background :DCAMKL1 or doublecortin-like kinase 1 contains two N-terminal doublecortin domains (which bind microtubules and regulate microtubule polymerization), a C-terminal serine/threonine protein kinase domain (which shows substantial homology to Ca2+/calmodulin-dependent protein kinase), and a serine/proline-rich domain in between the doublecortin and the protein kinase domains (which mediates multiple protein-protein interactions) (1). DCAMKL1 is a microtubule-associated kinase that can undergo autophosphorylation. DCAMKL1 has microtubule-polymerizing activity that is independent of its protein kinase activity (2). DCAMKL1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis.
References :
1. Ohmae, S. et.al: Molecular identification and characterization of a family of kinases with homology to Ca(2+)/calmodulin-dependent protein kinases I/IV. J. Biol. Chem. 281: 20427-20439, 2006
2. Lin, P. T. et,al: DCAMKL1 encodes a protein kinase with homology to doublecortin that regulates microtubule polymerization. J. Neurosci. 20: 9152-9161, 2000.
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Research Areas :Cardiovascular Disease, Neurobiology, PKA/PKC Pathway, Ser/Thr Kinases, Neurobiology, Cardiovascular Disease, PKA/PKC Pathway, Ser/Thr Kinases