DCAMKL1, Active (D14-10H)
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Description :Full-length recombinant human DCAMKL1 was expressed by baculovirus in Sf9 cells using an N-terminal his tag.
Species :Human
Tag :His tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_004734
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~85 kDa
Gene Aliases :DCLK1; DCDC3A; DCLK; KIAA0369
Scientific Background :DCAMKL1 or doublecortin-like kinase 1 contains two N-terminal doublecortin domains (which bind microtubules and regulate microtubule polymerization), a C-terminal serine/threonine protein kinase domain (which shows substantial homology to Ca2+/calmodulin-dependent protein kinase), and a serine/proline-rich domain in between the doublecortin and the protein kinase domains (which mediates multiple protein-protein interactions) (1). DCAMKL1 is a microtubule-associated kinase that can undergo autophosphorylation. DCAMKL1 has microtubule-polymerizing activity that is independent of its protein kinase activity (2). DCAMKL1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis.
References :
1. Ohmae, S. et.al: Molecular identification and characteri-zation of a family of kinases with homology to Ca(2+) /calmodulin-dependent protein kinases I/IV. J. Biol. Chem. 281: 20427-20439, 2006.
2. Lin, P. T. et,al: DCAMKL1 encodes a protein kinase with homology to doublecortin that regulates microtubule polymerization. J. Neurosci. 20: 9152-9161, 2000.
Product Sheets (By Lot #) :
Research Areas :Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, Ser/Thr Kinases