DMPK, Active(D07-10G)
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Description :Recombinant full-length human DMPK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_004409
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.activity.gif)
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.gel.png)
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~105kDa
Gene Aliases :DM, DM1, DMK, MDPK, DM1PK, MT-PK
Scientific Background :DMPK (or Myotonic Dystrophy Protein Kinase) is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. DMPK protein has a leucine-rich domain at the N-terminus, a serine/threonine kinase domain in the middle, and a hydrophobic region at the C-terminus. The N-terminus of DMPK plays an important role in DMPK kinase activity, and the C-terminus of DMPK determines the intracellular localization of the protein (1). Myotonic dystrophy type 1 is an autosomal dominant disease caused by a trinucleotide repeat-expansion, cytosine-thymine-guanine (CTG)n, in the 3' untranslated region of DMPK (2).
References :
1. Sasagawa, N. et al: Overexpression of human myotonic dystrophy protein kinase in Schizosaccharomyces pombe induces an abnormal polarized and swollen cell morphology. J Biochem. 2003 Oct;134(4):537-42.
2. Salvatori, S. et al: Decreased expression of DMPK: correlation with CTG repeat expansion and fibre type composition in myotonic dystrophy type 1. Neurol Sci. 2005 Oct;26(4):235-42.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cardiovascular Disease, Ser/Thr Kinases, Cancer, Cardiovascular Disease, Ser/Thr Kinases