Cy3 Labeled Negative Control siRNA (S02-910N)
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Description : beta-Actin Positive Control siRNA is a synthetic siRNA duplex designed to knock-down human beta-Actin mRNA expression. The synthetic siRNAs (5’-UGA AGA UCA AGA UCA UUG CdTdT-3’ and 5’-GCA AUG AUC UUG AUC UUC AdTdT-3’) enable qualitative assessment of knockdown and for optimizing RNAi experiments.
Purity : Cy3 Negative Control siRNA duplex resolves as a single band via PAGE
Format : Lyophilized powder
Reconstitution Protocol : Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Spectral Information : Filter: Cy3/TRITC
Excitation max (lambda max): 550 nm
Emission max (lambda max): 570 nm.
Storage and Stability : The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
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