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CXCL14 (BRAK)(M842-40N)

CXCL14 (BRAK)(M842-40N)

  • $193.00


Description :Recombinant Human CXCL14 (BRAK) was expressed in E. coli cells.

Species :Human

Expression System:E.coli

Genbank Number :O95715

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70 oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight :9.6 kDa

Gene Aliases :Chemokine BRAK, MIP-2G, Small-inducible cytokine B14.

Reconstitution Protocol :Reconstitute in sterile 4mM HCl at 100µg/mL (containing at least 0.1% human or bovine serum albumin).

Scientific Background :Chemokine (CXC motif) ligand 14, also known as breast and kidney-expressed chemokine (BRAK), is a CXC chemokine that is a potent chemoattractant for neutrophils but not for T-cells, B-cells, monocytes or granulocytes. Mature CXCL14 has many conserved features of the CXC family and is 30% homologous to the MIP-α and MIP-beta sequences, but differs in that it has an additional five amino acid sequence in between the third and fourth cysteines and a short N-terminus. It is usually highly expressed near its fibroblast source and in epidermal fibroblasts and keratinocytes of skin, but is absent from cancer cells.

Sample Cell Assay Data :The ED50 is 0.25-1.25 ng/mL as determined by its ability to induce calcium flux in activated monocytes.

Purity :Recombinant Human CXCL14 (BRAK) resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.

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Research Areas :Cancer