CXCL12 (SDF-1 beta)(S821-40BN)

CXCL12 (SDF-1 beta)(S821-40BN)

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Description :Recombinant Human CXCL12 (SDF-1 beta) was expressed in E. coli cells.

Species :Human

Expression System:E.coli

Genbank Number :P48061

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight :8.5 kDa

Gene Aliases :Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, IRH, Pre-B cell growth-stimulating factor, PBSF. Recombinant Human Stromal Cell-Derived Factor-1 Beta (CXCL12).

Reconstitution Protocol :A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Scientific Background :A member of the CXC chemokine family, CXCL12 is strongly chemotactic for T-, B-, and cancer cells. Known to exert its effects mainly through binding to the CXCR4 receptor, CXCL12 plays an important role in morphogenesis, angiogenesis, and cancer metastasis. CXCL12 also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for CXCL12. Encoded by a single gene, SDF-1 alpha (89 amino acids) and SDF-1 beta (93 amino acids) are derivatives of an alternative splicing event. SDF-1α and SDF-1β have identical amino acid sequences, differing only, by the presence of four extra amino acids at the C-terminus of the β form. SDF-1 beta is constitutively expressed by stromal cells.

Sample Cell Assay Data :Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentration range of 5.0-40 ng/Ml.

Purity :Recombinant Human CXCL12 (SDF-1 beta) resolved on a 17% SDS-PAGE gel under non-reducing conditions and stained with Coomassie Brilliant Blue G-250.

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Research Areas :Cancer