LwCas13a, Active (CS13A-E321H)

LwCas13a, Active (CS13A-E321H)

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Description : Recombinant Leptotrichia wadei LwCas13a (1-1152) was expressed in E. coli with a C-terminal His tag.

Species : Leptotrichia wadei

Tag : HIS tag

Expression System: E.coli

Sequence :1-1152

Genbank Number :  WP_021746774

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Activity: Sample Purity Data. For specific information on a given lot, see the related technical datasheet.

Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. 

Always include RNase inhibitor at manufacturer recommended concentrations in Cas enzyme reactions.

Molecular Weight : 130 kDa

Gene Aliases : CRISPR-associated endoribonuclease Cas13a, LwC2c2, LwaCas13a, EndoRNase, Uniprot accession U2PWF1.

Scientific Background : Cas13a is RNA-guided RNA endonuclease that belongs to class 2 type VI CRISPR-Cas system (1). Cas13a possess both RNA recognition and cleavage activities and cleaves RNA through conserved basic residues within its two HEPN domains (2). Interestingly, activated Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner. This property of Cas13a enzyme has been leveraged for the development of sensitive and robust nucleic acid detection diagnostic tool for clinical samples. CRISPR-Cas13a system provides opportunity for the development of clinical research in precision oncology (3).

References :

  1. East-Seletsky A, et al: Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 2016, 538:270–3.
  2. Abudayyeh O, et al: C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 2016, 353:5573.
  3. Zhang J, You Y. CRISPR-Cas13a system: A novel approach to precision oncology. Cancer Biol Med. 2020, 17(1):6-8.

Product Sheets (By Lot #) :

X3385-3