CPB (carboxypeptidase B), Active (C560-31N)
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Description : Recombinant rat tag-free CPB (109-end) was expressed in yeast cells.
Expression System : Yeast cells
Sequence : 109-end
Genbank Number :
Sample Data :
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Enzyme Commission Number : EC 220.127.116.11
Gene Aliases : CPB1; CPB2; Carboxylase B
Formulation : Recombinant protein stored in 50mM Tris-HCl, pH 8, 200mM NaCl, 30% glycerol.
Storage, Stability and Shipping : Store product at –20oC for up to 1 year. Aliquot enzymes to avoid freeze / thaw cycles.
Applications : In vitro Proteolysis
Digestion Conditions : Catalytic pH range: 5.0~12.0; maximum temperature: 60oC
Scientific Background : Carboxypeptidase B (CPB) is a metal-containing pancreatic endopeptidase that catalyzes the successive release of basic carboxy-terminal residues, such as Arg and Lys. CPB also targets other amino acids, albeit at a slower rate. These secondary target residues include valine, leucine, isoleucine, asparagine, glycine, and glutamine. CPB depends on Zn2+ to promote enzymatic activity. Conversely, the enzyme is inhibited by some heavy metals, including Cu2+, Hg2+; metal chelating agents such as EDTA and competing Arg and Lys residues. CPB functions between pH 5-12, with an optimal pH of 8. Enzyme activity also increases with rises in temperature. CPB is used in the production of recombinant human insulin and monoclonal antibody/IgG1 (mAb) processing.
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