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CDK9/CyclinK, Active(C40-10G)

CDK9/CyclinK, Active(C40-10G)

  • $226.00


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Description :Recombinant full-length human CDK9 and CyclinK were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

Species :Human

Tag :GST tags

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_001261

Genbank Number 2 :BC015935

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~68 kDa and CyclinK~67 kDa

Gene Aliases :CDC2L4, C-2k, TAK, PITALRE; CCNK, CPR4, MGC9113

Scientific Background :CDK9/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family. CDK9 is closely related to cdc28 and cdc2 and is an important regulator of the cell cycle (1). CDK9 is a component of the multiprotein complex TAK/P-TEFβ. CDK9 can modulate RNA polymerase II-directed transcription by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. CDK9 also interacts with the HIV-1 Tat protein which suggested a possible involvement of this protein in AIDS (2).

References :
1. Yang, Z. et al: The 7SK small nuclear RNA inhibits the CDK9/cyclin T1 kinase to control transcription. Nature, 2001; 414: 317-322.

2. Bullrich, F. et al: Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27-Kip1, to regions involved in human cancer. Cancer Res. 1995; 55: 1199-1226.

Product Sheets (By Lot #) :

N079-2.pdf

N354-1.pdf

L2159-9.pdf

E3115-9.pdf

Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases, Cancer, Cell Cycle, Ser/Thr Kinases