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Description :Recombinant human CDK5P25 (CDK5R1) (98-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Genbank Number :NM_003885
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~49 kDa
Gene Aliases :p25, CDK5R1, CDK5R, NCK5A, p23
Scientific Background :CDK5P25 (cyclin-dependent kinase 5, regulatory subunit 1) is a neuron-specific activator of CDK5 and is required for proper development of the central nervous system (1). The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form of the protein which relocates from the cell periphery to nuclear and perinuclear regions. CDK5P25 regulates CDK5 activity by prolonging its activation and changing its cellular location and this may be important in pain signaling (2). The CDK5P25 accumulates in the brain neurons of patients with Alzheimer's disease and this accumulation correlates with an increase in CDK5 kinase activity which may result in aberrantly phosphorylated forms of the microtubule-associated protein tau.
1. Tsai, L.-H.et.al: p35 is a neural-specific regulatory subunit of cyclin-dependent kinase 5. Nature 371: 419-423, 1994.
2. Pareek, T. K.et.al: Cyclin-dependent kinase 5 activity regulates pain signaling. Proc. Nat. Acad. Sci. 103: 791-796, 2006.
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Research Areas :Cancer, Cell Cycle, Cancer, Cell Cycle, Ser/Thr Kinases