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CDK4/CyclinD1, Active(C31-10G)

CDK4/CyclinD1, Active(C31-10G)

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Description :Recombinant full-length human CDK4 and CyclinD1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

Species :Human

Tag :GST tags

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_000075

Genbank Number 2 :NM_053056

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~57 kDa and CyclinD1 ~61 kDa

Gene Aliases :CDK4: CMM3; PSK-J3; MGC14458 CyclinD1: BCL1, PRAD1, U21B31, D11S287E

Scientific Background :CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of RB protein leading to an active transcriptional repression of E2F complex (1). CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 (2).

References :
1. Harbour, J W. et al: Cdk phosphorylation triggers sequential intramolecular interactions that progressively block Rb functions as cells move through G1. Cell 98: 859-869, 1999.

2. Matsuura, I. et al: Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430: 226-231, 2004.

Product Sheets (By Lot #) :

R219-1.pdf

E178-2.pdf

R2640-7.pdf

Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases, Cancer, Cell Cycle, Ser/Thr Kinases