CDK2/CyclinA1, Active(C29-10BG)

CDK2/CyclinA1, Active(C29-10BG)

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Description :Recombinant full-length human CDK2 and CyclinA1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_001798

Genbank Number 2 :BC036346

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~58 kDa and CyclinA1 ~81 kDa

Gene Aliases :CDK2: p33(CDK2) CyclinA1: CCNA1

Scientific Background :CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B) (1). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition (2).

References :
1. Levkau, B. et al: Cleavage of p21 (Cip1/Waf1) and p27 (Kip1) mediates apoptosis in endothelial cells through activation of Cdk2: role of a caspase cascade. Molec. Cell 1: 553-563, 1998.

2. Huang, H. et al: CDK2-dependent phosphorylation of FOXO1 as an apoptotic response to DNA damage. Science 314: 294-297, 2006.

Product Sheets (By Lot #) :

E359-2.pdf

E108-2.pdf

Y1115-1.pdf

C2091-5.pdf

E3260-9.pdf

Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases, Cancer, Cell Cycle, Ser/Thr Kinases