CDC7/DBF4, Active(C26-10G)

CDC7/DBF4, Active(C26-10G)

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Description :Recombinant full-length human CDC7 and DBF4 proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tags.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_003503

Genbank Number 2 :NM_006716

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :CDC7 ~94kDa and DBF4 ~125kDa

Gene Aliases :CDC7: CDC7L1; HsCDC7; Hsk1; huCDC7; MGC117361; MGC126237; MGC126238; DBF4: ASK; CHIF; DBF4A; ZDBF1

Scientific Background :CDC7 is a cell division cycle 7 homolog protein that is critical for the G1/S transition and is also essential for initiation of DNA replication as cell division occurs. CDC7 is expressed in many normal tissues, but the overexpression of CDC7 may be associated with neoplastic transformation for some tumors and transformed cell lines (1). CDC7/DBF4 kinase promotes S phase by alleviating an inhibitory activity in Mcm4 that evolved to integrate several protein kinase (2).

References :
1. Hess, G. F. et.al: A human homolog of the yeast CDC7 gene is overexpressed in some tumors and transformed cell lines. Gene 211: 133-140, 1998.

2. Sheu, Y.-J. et.al: The Dbf4-Cdc7 kinase promotes S phase by alleviating an inhibitory activity in Mcm4. Nature 463: 113-117, 2010.

Product Sheets (By Lot #) :

I363-1.pdf

B2013-11.pdf

Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases, Cancer, Cell Cycle, Ser/Thr Kinases