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CAMKK1, Active(C17-18G)

CAMKK1, Active(C17-18G)

  • $226.00


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Description :Recombinant full-length rat CAMKK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Rat

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :L42810

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~88 kDa

Gene Aliases :CaMKK1; CaMKK alpha; CaM-kinase IV kinase

Scientific Background :CAMKK1 or CAMKKalpha is a Ca(2+)/calmodulin-dependent protein kinase that activates CAM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively. Recent studies have shown that the activity of CAMKK1 is decreased upon phosphorylation by cAMP-dependent protein kinase (PKA) (1) The CAMKKalpha has been identified in intact cells as AMPKKs, predicting a significant role for this kinase in regulating AMPK activity in vivo. It has been shown that 2-deoxyglucose- and ionomycin-stimulated AMPK activity is substantially reduced in HeLa cells transfected with small interfering RNAs specific for CAMKKalpha (2).

References :
1. Okuno, S. et al: Regulation of Ca(2+)/calmodulin-dependent protein kinase kinase alpha by cAMP-dependent protein kinase: I. Biochemical analysis. J Biochem (Tokyo). 2001 Oct;130(4):503-13.

2. Hurley, R L. et al: The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases. J Biol Chem. 2005 Aug 12;280(32):29060-6.

Product Sheets (By Lot #) :

T990-1.pdf

Research Areas :Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, Ser/Thr Kinases, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, PKA/PKC Pathway, Ser/Thr Kinases