Anti-Phospho-Cyclin B1 (Ser126)(C79-365R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the human Cylcin B1 protein phosphorylated at Serine126
Cited Applications :ELISA, IP, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human, Rat, Dog, Chimpanzee, Mouse and Hamster
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 12-131 of Human Cyclin B1 protein
Purification :Immunoaffinity chromatography followed by solid phase adsorption
Stability :1yr at –20oC from date of shipment
Sample Data :Western blot analysis using Anti-phospho-Cyclin B1 antibody (1:100) to detect phosphorylated human Cyclin B1 (~48kDa). lane 1 - Hela (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). Each lane contains approximately 50 µg of lysates
Scientific Background :The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
References :
1. Giordano, A., et al.: A 60 kd cdc-2 associated polypeptide complexes with the E1A proteins of adenovirus-infected cells. Cell. 1989: 58; 981-990.
2. Pines, J., et al.: Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B. Nature. 1990: 346; 760-763.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases