CK1 delta, Active(C65-10G)
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Description :Full length recombinant human CK1 delta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_001893
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~72 kDa
Gene Aliases :CSNK1D, HCKID, CK1D
Scientific Background :CK1δ is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. CK1δ is a regulator of Yes-associated protein (YAP) transcription coactivator which is a key regulator of organ size and a candidate human oncogene. CK1δ is activated by CCK2R and this then phosphorylates PKD2 at Ser244. Phosphorylation of PKD2 leads to its nuclear accumulation and efficient phosphorylation of nuclear PKD2 substrates in human gastric cancer cells (1). CKIδ can phosphorylate in vitro deoxycytidine kinase (dCK) which is a key enzyme in the salvage of deoxynucleosides. Phosphorylation of dCK by CKIδ in vitro correlates with increased activity of this enzyme (2).
References :
1. von Blume J. et al: Phosphorylation at Ser244 by CK1 determines nuclear localization and substrate targeting of PKD2. EMBO J. 2007 Nov 14;26(22):4619-33.
2. Smal, C. et al: Casein kinase 1delta activates human recombinant deoxycytidine kinase by Ser-74 phosphorylation, but is not involved in the in vivo regulation of its activity. Arch Biochem Biophys. 2010 Oct 1;502(1):44-52.
Product Sheets (By Lot #) :
Research Areas :Cancer, Inflammation, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling, Cancer, Neurobiology, Inflammation, NfkB Pathway, WNT Signaling, Ser/Thr Kinases