CK1 alpha 1, Active(C64-10G)
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Description :Full length recombinant human CK1 alpha 1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_001892
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~62 kDa
Gene Aliases :CSNK1A1, CK1, HLCDGP1, PRO2975, CK1A1
Scientific Background :CK1α1 is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes. CK1α1 can regulate Smo cell surface accumulation and activity in response to hedgehog. CK1α1 phosphorylate Smo at several sites and phosphorylation-deficient forms of Smo fail to accumulate on the cell surface and are unable to transduce the hedgehog signal (1). CK1α1 dynamically associates with the CBM complex on T cell receptor engagement to participate in cytokine production and lymphocyte proliferation. CK1α1 can form complex with MDM2 which then regulates the stability of p53 and E2F-1 transcription factors (2).
References :
1. Jia, J. et al: Hedgehog signalling activity of Smoothened requires phosphorylation by protein kinase A and casein kinase I. Nature 432: 1045-1050, 2004.
2. Huart, A S. et al: CK1alpha plays a central role in mediating MDM2 control of p53 and E2F-1 protein stability. J Biol Chem. 2009 Nov 20;284(47):32384-94.
Product Sheets (By Lot #) :
E3307-8.pdfResearch Areas :Cancer, Inflammation, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling, Cancer, Neurobiology, Inflammation, NfkB Pathway, WNT Signaling, Ser/Thr Kinases