Anti-IKK alpha(C51-63BR)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the IKK alpha protein and has no cross response to IKK beta or IKK gamma
Cited Applications :WB, ELISA, ICC, IF
Cross Reactivity :Human
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :IKK alpha antibody was raised against a peptide corresponding to amino acids 699 to 715 of human IKK alpha, which differs from corresponding murine sequence by one amino acid.
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:500 dilution.
Sample Data :Immunocytochemistry of IKK alpha in HeLa cells with IKK alpha antibody at 10 ug/ml.
Scientific Background :IKKalpha is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex (1). IKKalpha is an essential regulator of NF-kappa-B-dependent gene expression through control of promoter-associated histone phosphorylation after cytokine exposure (2). IKKalpha is a critical component of the cytoplasmic transductional-transcriptional processor leading to induction of IFN alpha production. IKKalpha is also involved in the epidermis where it antagonizes mitogenic and angiogenic signals and represses tumor progression and metastases.
References :
1. Anest, V.et.al: A nucleosomal function for I-kappa-B kinase-alpha in NF-kappa-B-dependent gene expression. Nature 423: 659-663, 2003.
2. Hoshino, K. et.al: I-kappa-B kinase-alpha is critical for interferon-alpha production induced by Toll-like receptors 7 and 9. Nature 440: 949-953, 2006.
Product Sheets (By Lot #) :
Research Areas :Cancer, Inflammation, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling, Inflammation, AKT/PKB Pathway, p38 Pathway, Apoptosis/Autophagy