Anti-CHK2(C48-60M)

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Description :Mouse Monoclonal Antibody

Species :Mouse

Tag :

Expression System:

Sequence :

Specificity :Recognizes the CHK2 protein

Cited Applications :WB

Cross Reactivity :Western blot CHK2 from human and mouse cells

CHK2 from other species may also be detectable.

Host / Isotype / Clone# :Mouse, IgG1, 7F9

Immunogen :Human full length recombinant CHK1

Purification :Affinity Chromatography

Stability :Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Representative western blot (1:1000) with Anti-CHK2 using 20 ng of GST-tagged human full length recombinant CHK1 and CHK2.

Sample Data :Representative western blot (1:1000) with Anti-CHK2 using 3 µg, 10 µg, 30µg of 293T cell lysate.

Scientific Background :CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner (1). Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization (2).

References :
1. Matsuoka, S. et al: Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998 Dec 4;282(5395):1893-7.

2. Chehab NH. et al: Chk2/hCds1 functions as a DNA damage checkpoint in G(1) by stabilizing p53. Genes Dev. 2000 Feb 1;14(3):278-88.

Product Sheets (By Lot #) :

D339-4.pdf

Research Areas :Cell Cycle, Ser/Thr Kinases, Cell Cycle, Ser/Thr Kinases