CDK9/CyclinT2, Active(C40-18BG)
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Description :Recombinant full-length human CDK9 and CyclinT2 proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_001261
Genbank Number 2 :NM_001241
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~68 kDa/105 kDa
Gene Aliases :CDK9: CDC2L4, C-2k, TAK, PITALRE
CyclinT2: CCNT2; CYCT2
Scientific Background :CDK9/Cyclin T2 is a member of the cyclin-dependent protein kinase (CDK) family which are known to be important regulators of cell cycle progression. Cyclin T2 and its kinase partner CDK9 are found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin has been reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein (1). The transcription-dependent interaction of P-TEFb with 7SK may therefore contribute to an important feedback loop modulating the activity of RNA Pol II (2).
References :
1. Peng, J.et.al: Identification of multiple cyclin subunits of human P-TEFb. Genes Dev. 12: 755-762, 1998.
2. Nguyen, V. T.et.al: 7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes.Nature 414: 322-325, 2001.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases, Cancer, Cell Cycle, Ser/Thr Kinases