CDK3/CyclinE2, Active(C30-10BG)
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Description :Recombinant full-length human CDK3 and Cyclin E2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Species :Human
Tag :GST tags
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_001258
Genbank Number 2 :NM_057749
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :CDK3 ~60 kDa and CyclinE2 ~72 kDa
Gene Aliases :CDK3: None; CyclinE2: CCNE1
Scientific Background :CDK3/CyclinE is a member of the cyclin-dependent protein kinase family and promotes entry into S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. The CDK3 gene has been mapped to chromosomal location of 17q22-qter, telomeric to the BRCA1 gene by somatic cell hybrids analysis (1). The presence of a single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported (2).
References :
1. Bullrich, F. et al: Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27-Kip1, to regions involved in human cancer. Cancer Res. 55: 1199-1226, 1995.
2. Ye, X. et al: A premature-termination mutation in the Mus musculus cyclin-dependent kinase 3 gene. Proc. Nat. Acad. Sci. 98: 1682-1686, 2001.
Product Sheets (By Lot #) :
Research Areas :Ser/Thr Kinases, Cell Cycle, Cancer