Anti-p27KIP1 (CDKN1B)(C28-362R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the p27 protein from human tissue
Cited Applications :ELISA, IP, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human
Host Isotype / Clone# :Rabbit, Antiserum
Immunogen :The antibody was produced against full length recombinant human p27 protein
Purification :Delipidation and defibrination
Stability :1yr at –20oC from date of shipment
Sample Data :Western blot using Anti-p27KIP1 (CDKN1B) (1:500 dilution) shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead). 
Scientific Background :KIP1 (cyclin-dependent kinase inhibitor 1B) is a kinesin-related motor protein required for mitotic spindle assembly and chromosome segregation (1). Many tumorigenic processes modulate cell-cycle progression by regulating the levels of the cyclin-dependent kinase inhibitor KIP1. KIP1 binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (2). The phosphorylation- and ubiquitination-dependent proteolysis of KIP1 is implicated in control of the G1-S transition in the cell cycle. KIP1 is critical for retinoblastoma protein (Rb)-induced cellular proliferative senescence.
References :
1. Hoyt, M A. et al: Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly. J. Cell Biol. 1992 118(1):109-120.
2. Carrano,A C. et al: SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27. Nat. Cell Biol. 1999; 1, 193-199
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle