Anti-p21CIP1 (p21/WAF1)(C27-61M)
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Description :Mouse Monoclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the p21CIP1 (p21/WAF1) protein
Cited Applications : WB, ELISA, IP
Cross Reactivity :Human
Host / Isotype / Clone# :Mouse, IgG
Immunogen :Produced by repeated immunizations with recombinant human p21 fusion protein.
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Anti-p21CIP1 (p21/WAF1) detects human p21 by western blot. 10 µg of a HeLa whole cell lysate was loaded per lane and incubated with a 1:1,000 of Anti-p21CIP1 (p21/WAF1). A single band corresponding to human p21WAF1 is detected at ~21 kDa.
Sample Data :Immunohistochemistry of Anti-p21CIP1 (p21/WAF1) antibody 1:250. Tissue: human brain. Co-stained with YFP and Sox2 antibodies. Localization: p21 is nuclear and cytoplasmic. Staining: p21CIP1 as precipitated blue with Cy5, YFP as precipitated green with Cy2, and Sox2 as precipitated red with Cy3. z-stacks from confocal expressed as one composite focal plane.
Scientific Background :CIP 1 (Cyclin-Dependent Kinase Inhibitor 1A) regulates cell cycle progression, terminal differentiation, and apoptosis (1). CIP1 is induced by p53 and is a potent inhibitor of cyclin-dependent kinase (CDK) activity. DNA damage leads to increased expression of CIP1 in cyclin E-containing complexes and to an associated decrease in cyclin-dependent kinase activity. CIP1 is a critical downstream effector in the p53-specific pathway of growth control in mammalian cells (2).
References :
1. el-Deiry, W S. et al: WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res. 1994;54(5):1169-74.
2. Wagner, M. et al: Cyclin-dependent kinase-inhibitor 1 (CDKN1A) in the squamous epithelium of the oropharynx: possible implications of molecular biology and compartmentation. Anticancer Res. 2001; 21(1A):333-45.
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Research Areas :Cancer, Cell Cycle, Cancer, Cell Cycle