2019-nCoV Neutralization ELISA Kit (C19SD-N887)
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Overview : The 2019-nCoV Neutralization ELISA Kit is an enzyme-linked immunosorbent assay for the detection of antibodies or other proteins (molecular) that can block the binding of SARS-CoV-2 S1 protein and human ACE2 in serum or other biological samples. Based on indirect ELISA principles, the 2019- nCoV Neutralization ELISA employs recombinant human ACE2 protein coated on a 96-well plate. Controls and samples (with necessary dilution) are mixed with 2019-nCOV S1 protein RBD-HRP and then pipetted into the wells; the target neutralizing antibodies or proteins in the controls and samples will partially or completely block binding of the S1 protein RBD-HRP to the immobilized ACE2 protein. After washing away the unbound 2019-nCOV S1 protein RBD-HRP, a colorimetric substrate solution (TMB) is pipetted to the wells. The absorbance of the color at 450 nm is measured. The intensity of color development in the wells is proportional to the amount of target protein bound.
Components :
| Catalog # | Component Name | Size | Storage |
| C19SD-G241DH | 2019-nCoV S1 protein RBD-HRP Cconjugate |
8 mL x 1 vial |
Store kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening. |
| CP02-A51C2 | (ACE2) 19-740 aa coated plate | 1 plate | |
| CS02-506 |
One step TMB Substrate |
10 mL x 1 vial | |
| E01-09 |
nCoV ELISA sample dilution buffer |
10 mL x 1 vial | |
| SS01-09 |
Stop Solution |
6 mL x 1 vial | |
| C19N-NEG |
Nab Negative Controls |
150 uL x 1 vial | |
| C19N-POS | Nab Positive Controls | 150 uL x 1 vials | |
| WB20-09 | Wash buffer, 20X | 15 mL x 1 vials |
Representative Data : In order to evaluate the presence of Neutralizing antibodies or proteins in a biological sample, the O.D. value of the sample must be compared to the values obtained for the positive and negative controls
Assay Work Flow : Click here to see image
Technical Manual:
CoA Sheet :
SDS :
References :
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.
2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.
3. Long Q, et al: Antibody responses to SARS-CoV-2 in patients with COVID-19 Quan-Xin Long. Nature Medicine. 2020, 29 April.
4. Ma H, et al: Serum IgA, IgM, and IgG responses in COVID-19. Cell Mol Immunol. 2020, 17: 773-775.
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