2019-nCoV Neutralization ELISA Kit (C19SD-N887)

2019-nCoV Neutralization ELISA Kit (C19SD-N887)

  • $620.00


Overview : The 2019-nCoV Neutralization  ELISA  Kit  is  an  enzyme-linked  immunosorbent  assay  for  the  detection of  antibodies or other proteins (molecular) that can block the binding of SARS-CoV-2 S1 protein and  human ACE2 in  serum  or  other biological samples.  Based  on  indirect  ELISA  principles,  the  2019-  nCoV Neutralization ELISA employs recombinant human ACE2  protein  coated  on  a  96-well  plate. Controls and samples (with necessary dilution) are mixed  with  2019-nCOV  S1  protein  RBD-HRP  and  then pipetted into the wells; the target  neutralizing antibodies or proteins  in  the  controls  and  samples will partially or  completely  block  binding  of  the  S1  protein  RBD-HRP  to  the  immobilized  ACE2  protein.  After  washing  away  the   unbound   2019-nCOV   S1  protein   RBD-HRP,   a   colorimetric substrate   solution   (TMB)    is    pipetted    to    the    wells.    The absorbance  of  the  color  at  450  nm   is measured. The intensity of color development in the wells is proportional to  the amount  of  target protein bound.

Components :

Catalog # Component Name Size Storage
C19SD-G241DH 2019-nCoV S1 protein RBD-HRP Cconjugate
8 mL x 1 vial
Store kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening.
CP02-A51C2 (ACE2) 19-740 aa coated plate 1 plate
One step TMB Substrate
10 mL x 1 vial
nCoV ELISA sample dilution buffer
10 mL x 1 vial
Stop Solution
6 mL x 1 vial
Nab Negative Controls
150 uL x 1 vial
C19N-POS Nab Positive Controls 150 uL x 1 vials
WB20-09 Wash buffer, 20X 15 mL x 1 vials

Representative Data : In order to evaluate the presence of Neutralizing antibodies or proteins in a biological sample, the O.D. value of the sample must be compared to the values obtained for the positive and negative controls

Assay Work Flow : Click here to see image

Technical Manual:

CoA Sheet :


References :
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.
2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.
3. Long Q, et al: Antibody responses to SARS-CoV-2 in patients with COVID-19 Quan-Xin Long. Nature Medicine. 2020, 29 April.
4. Ma H, et al: Serum IgA, IgM, and IgG responses in COVID-19. Cell Mol Immunol. 2020, 17: 773-775.

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