2019-nCoV Spike protein S1 (Δ69-70) (C19S1-G234H)

2019-nCoV Spike protein S1 (Δ69-70) (C19S1-G234H)

  • $100.00


Description: Recombinant 2019-nCoV Spike protein S1 (Δ69-70) (16-685) was expressed in CHO cells using a C-terminal His- tag.

Species: SARS-CoV-2

Tag: HIS tag

Expression System: CHO Cells

Sequence: 319-541

Genbank Number: MN908947

Purity: The purity of nCoV-S1 (Δ69-70) was determined to be >90% by densitometry, approx MW 130 kDa (calculated MW~76 kDa).

Activity: Binding of ACE2 (19-740) Protein (A51C2-G341F) to immobilized 2019-nCoV spike protein S1 (Δ69-70) (C19S1-G234H) was determined by functional ELISA.

Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight: MW 130 kDa (calculated MW~76 kDa).

Gene Aliases : 2019-nCoV s1, SARS-CoV-2 spike S1, SARS-CoV-2 S1, novel coronavirus spike s1, nCov spike s1, coronavirus spike S1.

Scientific Background: Novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world since 2020 (1). The spike glycoprotein (S) of coronavirus, a type I transmembrane protein containing two subunits, S1 and S2 is known to bind with host cells through the interaction with angiotensin-converting enzyme 2 (ACE2) and facilitate viral entry into the host cell (2). SARS-CoV-2 variant carrying Δ69-70 has been detected in human cases in Denmark and UK. Mutation Δ69-70 has been associated with increased viral infectivity. As new variants displace the first-wave virus, it is pivotal to evaluate their transmissibility, virulence and their possible tendency to escape antibody neutralization (2, 3).

References : 

1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Tada T, et al: Neutralization of viruses with European, South African, and United States SARS-CoV-2 variant spike proteins by convalescent sera and BNT162b2 mRNA vaccine-elicited antibodies. https://www.biorxiv.org/content/10.1101/2021.02.05.430003v1.

3. Kemp SA, et al: Recurrent emergence and transmission of a SARS-CoV-2 Spike deletion H69/V70. bioRxiv 2020.12.14.422555; doi: https://doi.org/10.1101/2020.12.14.422555

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