2019-nCoV Spike protein S1 (A222V) (C19S1-G233H)
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Description: Recombinant 2019-nCoV Spike protein S1 (A222V) (16-685) was expressed in CHO cells using a C-terminal His- tag.
Species: SARS-CoV-2
Tag: HIS tag
Expression System: CHO Cells
Sequence: 319-541
Genbank Number: MN908947
Purity: The purity of 2019-nCoV-S1 (A222V) was determined to be >90% by densitometry, approx. MW 130 kDa (calculated MW~76 kDa).
Activity: Binding of immobilized 2019-nCoV spike protein S1 (A222V) (C19S1-G233H) to ACE2 (19-740) Protein (A51C2-G341F) was determined by functional ELISA.
Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight: 130 kDa
Gene Aliases : 2019-nCoV s1, SARS-CoV-2 spike S1, SARS-CoV-2 S1, novel coronavirus spike s1, nCov spike s1, coronavirus spike S1.
Scientific Background: Novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world since 2020 (1). The spike glycoprotein (S) of coronavirus, a type I transmembrane protein containing two subunits, S1 and S2 is known to bind with host cells through the interaction with angiotensin-converting enzyme 2 (ACE2) and facilitate viral entry into the host cell (2). A variant of SARS-CoV-2 carrying A222V mutation in the spike protein, also designated as lineage B.1.177, originated in Spain and then spread rapidly across Europe. As new variants displace the first-wave virus, it is pivotal to evaluate their transmissibility, virulence and their possible tendency to escape antibody neutralization (3).
References :
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.
2. Lan J, et al: Crystal structure of the 2019-nCov spike receptor-binding domain bound with the ACE2 receptor. Nature. 2020, 581:215-220.
3. Harvey WT, et al: SARS-CoV-2 variants, spike mutations and immune escape. Nat Rev. Microbiol. 2021, 19:409–424.
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