2019-nCoV Spike protein S1  (Δ69-70, N439K, D614G) (C19S1-G231BH)

2019-nCoV Spike protein S1 (Δ69-70, N439K, D614G) (C19S1-G231BH)

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Description: Recombinant 2019-nCoV Spike protein S1 (Δ69-70, N439K, D614G) (16-685) was expressed in CHO cells using a C-terminal His- tag.

Species: SARS-CoV-2

Tag: HIS tag

Expression System: CHO Cells

Sequence: 16-685

Genbank Number: MN908947

Purity: Sample Purity Data. For specific information on a given lot, see the related technical datasheet.

Storage, Stability, and Shipping: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight:  76 kDa

Gene Aliases : 2019-nCoV s1, SARS-CoV-2 spike S1, SARS-CoV-2 S1, novel coronavirus spike s1, nCoV spike s1, coronavirus spike S1.

Scientific Background: Novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world since 2020 (1). The spike glycoprotein (S) of coronavirus, a type I transmembrane protein containing two subunits, S1 and S2 is known to bind with host cells through the interaction with angiotensin-converting enzyme 2 (ACE2) and facilitate viral entry into the host cell (2). ΔH69–V70 deletion in the spike protein has been associated with immune escape in immunocompromised individuals treated with convalescent plasma. SARS-CoV-2 with D614G mutation exhibit increased sensitivity to neutralizing antibodies and N439K mutation results in enhanced binding affinity to the hACE2 receptor. As new variants emerge, it is pivotal to evaluate their transmissibility, virulence and their possible tendency to escape antibody neutralization (3).

References : 

  1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.
  2. Lan J, et al: Crystal structure of the 2019-nCov spike receptor-binding domain bound with the ACE2 receptor. Nature. 2020, 581:215-220.
  3. Harvey WT, et al: SARS-CoV-2 variants, spike mutations and immune escape. Nat Rev. Microbiol. 2021, 19:409–424.

Product Sheets (By Lot #) :

J4015-1