Anti 2019-nCoV Spike Protein Neutralizing Nanobody (NBX20010)(C19S1-641HL)
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Description: Recombinant Llama VHH-Human IgG Antibody
Specificity: Recognizes the 2019-nCoV Spike protein
Cited Applications: ELISA. For high-sensitivity SARS-CoV-2 spike protein detection assays. Ideal working dilutions for each application should be empirically determined by the investigator.
Host / Isotype / Clone# : Llama VHH-Human IgG Fc
Stability:1yr at –20oC from date of shipment.
Scientific Background: The severe acute respiratory syndrome-related novel coronavirus SARS-CoV-2 has caused the pandemic of respiratory diseases (COVID-19) around the world in 2020 (1). The spike glycoprotein (S) of coronavirus belongs to the type I transmembrane protein containing two subunits, S1 and S2 (2), which is also known to be the key component to bind with host cells through the interaction with angiotensin-converting enzyme 2 (ACE2) (3). A receptor-binding domain (RBD) of S1 can recognize the cell surface receptor and the mutation of RBD could cause a higher motility rate (3).
References:
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.
2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.
3. Lan J, et al: Crystal structure of the 2019-nCov spike receptor-binding domain bound with the ACE2 receptor. bioRxiv. doi: https://doi.org/10.1101/2020.02.19.956235
Assay Data: Binding ability measured in a functional ELISA. Immobilized Anti 2019-nCoV Spike Protein Llama heavy-chain (C19S1-641HL) binds 2019-nCoV Spike protein RBD (C19SD-241H) that can be detected at picomol level using Anti 2019-nCoV Spike Protein hIgG Antibody (C19S1-61H).
Assay Data: Binding of Anti 2019-nCoV Spike Protein Llama heavy-chain (C19S1-641HL) to 2019-nCoV RBD (C19SD-G241H) determined by ELISA.
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