2019-nCoV RBD-N Protein Chimera (C19NSD-G242H)

2019-nCoV RBD-N Protein Chimera (C19NSD-G242H)

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Description: Recombinant 2019-nCoV’s N-terminal Spike S1 RBD (319-541) fused to Nucleocapsid (237-419) protein with a linker HSA (human serum albumin) was expressed in CHO cells using a C-terminal His tag.

Tag: C-Terminal HIS tag

Expression System: CHO cells

Sequence :RBD (319-541) / N protein (237-419)

Genbank Number :MN908947

Genbank Number 2 :QHD43423

Specific Activity: Binding of 2019-nCoV RBD-N Protein Chimera (C19NSD-G242H) to immobilized 2019-nCoV N-protein hIgG antibody (C19NP-60H) determined by ELISA using HRP-conjugated Anti 2019-nCoV Spike Protein hIgG antibody (C19S1-60DH) as the detection antibody.

Purity: The purity of the 2019-nCoV RBD-N Protein Chimera protein was determined to be >70% by SDS-PAGE quantitative densitometry.

Storage, Stability, and Shipping: Store product at –70oC. For optimal storage, aliquot targets into smaller quantities after centrifugation and store at the recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight: 122 kDa

Gene Aliases: 2019-nCoV s1, SARS-CoV-2 spike S1, SARS-CoV-2 S1, novel coronavirus spike s1, nCov spike s1, coronavirus spike S1; Nucleoprotein, Nucleocapsid protein, NC, Protein N

Scientific Background: The severe acute respiratory syndrome related novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world in 2020 (1). The spike glycoprotein (S) of coronavirus belongs to the type I transmembrane protein containing two subunits, S1 and S2 (2), which is also known to be the key component to bind with host cells through the interaction with angiotensin-converting enzyme 2 (ACE2) (3). N-protein is a potent antigen that provides basis for future vaccine and diagnostic kit development (4).

References :
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.

3.Lan J, et al: Crystal structure of the 2019-nCov spike receptor-binding domain bound with the ACE2 receptor. bioRxiv.doi:href="https://doi.org/10.1101/2020.02.19.956235">https://doi.org/10.1101/2020.02.19.956235

4.McBride R, et al: The coronavirus nucleocapsid is a multifunctional protein. Viruses. 2014, 6:2991-3018.

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