NSP14, Active (C19NS-E311H)
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Description: Recombinant SARS-CoV-2 (COVID-19) NSP14 Methyltransferase (5925-6452) was expressed in E. coli using a C-terminal His-tag.
Species: Virus
Tag: HIS tag
Expression System: E.coli
Sequence: 5925-6452
Genbank Number: QHD43415
Activity: Sample Activity Plot. For specific information on a given lot, see related technical datasheet.
Purity: Sample Purity Data. For specific information on a given lot, see the related technical datasheet.
Storage, Stability, and Shipping: Store product at –70oC. For optimal storage, aliquot targets into smaller quantities after centrifugation and store at the recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight: 60 kDa
Gene Aliases : NSP14: Non-structural protein 14
Scientific Background: Coronaviruses codes for a bifunctional non-structural protein 14 (NSP14) that is important for viral replication and transcription. NSP14 contains an N-terminal exo-ribonuclease (ExoN) domain for proofreading during viral replication and a C-terminal N-7 methyltransferase (N7-MTase) domain for mRNA capping. NSP14 associated with several viral proteins (1). It can bind to NSP10 and the resulting complex exhibits enhanced ExoN activity in vitro (2). It can also bind to the polymerase complex (NSP12/NSP8/NSP7) forming a complex that retains all associated enzymatic activities (3).
References :
1.Ma, Y. et al: Structural basis and functional analysis of the SARS coronavirus nsp14-nsp10 complex. PNAS, USA. (2015), 112(30):9436–9441.
2.Bouvet, M. et al: RNA 3′-end mismatch excision by the severe acute respiratory syndrome coronavirus nonstructural protein nsp10/nsp14 exoribonuclease complex. Proc Natl Acad Sci USA. (2012), 109:9372–9377.
3.Subissi, L. et al: One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities. Proc Natl Acad Sci USA. (2014), 111:E3900–E3909.
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