2019-nCoV Nucleocapsid protein CTD (C19ND-G241H)

2019-nCoV Nucleocapsid protein CTD (C19ND-G241H)

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Description :Recombinant 2019-nCoV Nucleocapsid (N) Protein C-terminal domain (237-419) was expressed in E. coli cells with a C-terminal His tag.

Species :Virus

Tag :HIS tag

Expression System: E.Coli

Sequence : 237-419

Genbank Number : QHD43423

Activity :Sample Activity Data. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight : 26 kDa

Gene Aliases : Nucleoprotein, N-protein, Nucleocapsid protein, NC, Protein N

Scientific Background : CoV N protein is an RNA-binding protein primarily involved in binding to the viral RNA genome during packaging of viral RNA into a helical ribonucleoproteins or nucleocapsid structure (1). In addition, N-protein regulates viral RNA synthesis during replication/transcription and modulates host-pathogen interactions, such as such as actin reorganization, host cell cycle progression, and apoptosis (2). N protein is abundantly expressed during infection and antibodies against N-protein have been detected in confirmed cases of Covid-19. Therefore, N-protein is a potent antigen that provides basis for future vaccine and diagnostic kit development (3).

References :

1. McBride R, et al:  The coronavirus nucleocapsid is a multifunctional protein. Viruses. 2014, 6:2991-3018.

2. Surjit M, et al:  The nucleocapsid protein of severe acute respiratory syndrome-coronavirus inhibits the activity of cyclin-cyclin-dependent kinase complex and blocks S phase progression in mammalian cells. J Biol Chem. 2006, 281:10669-10681

3. Zeng W, et al: Biochemical characterization of SARS-CoV-2 nucleocapsid protein. Biochem Biophys Res Commun. 2020, 527(3):618‐623

Product Sheets (By Lot #) :

J3942-5.pdf