2019-nCoV NSP8 Protein (C19N8-E541H)
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Description: Recombinant full-length viral NSP8 was expressed in E. coli using a C-terminal His tag.
Species: Virus
Tag: HIS tag
Expression System: E.coli
Sequence: Full Length
Genbank Number: QHD43415
Purity: Sample Purity Data. For specific information on a given lot, see the related technical datasheet.
Storage, Stability, and Shipping: Store product at –70oC. For optimal storage, aliquot targets into smaller quantities after centrifugation and store at the recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight: 22 kDa
Gene Aliases : NSP8: Non-structural protein 8
Scientific Background: Novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world in 2020 (1). Coronavirus RNA genome encodes a complex replication machinery consisting of 16 viral non-structural proteins (NSPs). NSP7 and NSP8 stabilize regions of NSP12 (RNA-dependent RNA polymerase; RdRp) involved in RNA binding and are essential for a highly active NSP12 polymerase complex. NSP7 has been suggested to confer RNA-binding properties to the trimeric polymerase complex (2). NSP8 has been shown to act as an oligo(U)-templated polyadenylyltransferase and a robust (mono/oligo) adenylate transferase (3). The NSP7/NSP8/NSP12 polymerase complex associates with NSP14 which is required to safeguard coronavirus replication fidelity (2).
References :
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.
2. Subissi L, et al: One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities. PNAS. U.S.A. 2014, 111: E3900–E3909.
3. Tvarogova J, et al: Identification and characterization of a human coronavirus 229E nonstructural protein 8-associated RNA 3'-terminal adenylyltransferase activity. Virol. 2019, 368: 779-782.
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