CD19 Protein(C19-35H)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Recombinant human CD19 (20-291) was expressed in CHO cells using a C-terminal His tag.
Species :Human
Tag :HIS
Expression System:CHO cells
Sequence :20-291
Genbank Number :P15391
Alternative Name(s) :B-lymphocyte antigen CD19; Differentiation antigen CD19; T-cell surface antigen Leu-12
Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :B-lymphocyte antigen CD19 is a type I transmembrane protein present on all B cells and is a dominant signaling component of mature B cells (1). CD19 acts as a critical coreceptor for B cell receptor (BCR) signal transduction by recruiting and amplifying the activation of Src-family protein tyrosine kinases such as Lyn and Fyn (1). CD19 also enhances BCR-induced signaling crucial for B cell expansion through recruitment and activation of PI3K and downstream AKT kinases (1,2). CD19-deficient humans and mice exhibit hypo-responsiveness to transmembrane signals, and weak T cell-dependent humoral responses, leading to an overall impaired immune response (1).
References :1. Wang, K., Wei, G. & Liu, D. CD19: a biomarker for B cell development, lymphoma diagnosis and therapy. Exp Hematol Oncol. 2012, 31:1.
2. Del Nagro, C.J. et al. CD19 function in central and peripheral B-cell development. Immunol Res. 2005, 31(2):119-31.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Observed MW ~50-65 kDa. Calculated MW ~31 kDa.
Product Sheets (By Lot #) :